文章简介

出版社:Sci Rep.

作   者:Liu A, Xue GH, Sun M, Shao HF, Ma CY, Gao Q, Gou ZR, Yan SG, Liu YM, He Y

编   号:10.1038/srep21704.

年   份:2016    点击量:480

文章摘要

Desktop three-dimensional (3D) printers (D3DPs) have become a popular tool for fabricating personalized consumer products, favored for low cost, easy operation, and other advantageous qualities. This study focused on the potential for using D3DPs to successfully, rapidly, and economically print customized implants at medical clinics. An experiment was conducted on a D3DP-printed anterior cruciate ligament surgical implant using a rabbit model. A well-defined, orthogonal, porous PLA screw-like scaffold was printed, then coated with hydroxyapatite (HA) to improve its osteoconductivity. As an internal fixation as well as an ideal cell delivery system, the osteogenic scaffold loaded with mesenchymal stem cells (MSCs) were evaluated through both in vitro and in vivo tests to observe bone-ligament healing via cell therapy. The MSCs suspended in Pluronic F-127 hydrogel on PLA/HA screw-like scaffold showed the highest cell proliferation and osteogenesis in vitro. In vivo assessment of rabbit anterior cruciate ligament models for 4 and 12 weeks showed that the PLA/HA screw-like scaffold loaded with MSCs suspended in Pluronic F-127 hydrogel exhibited significant bone ingrowth and bone-graft interface formation within the bone tunnel. Overall, the results of this study demonstrate that fabricating surgical implants at the clinic (fab@clinic) with D3DPs can be feasible, effective, and economical.

台式三维(3D)打印机(D3DPs)已成为制作个性化消费品的一种普及工具,因其低成本、易操作和其它优点备受青睐。本研究主要集中在医疗诊所使用D3DPs成功、迅速及经济打印定制移植物的可能性。利用兔模型在D3DP打印好的前交叉韧带外科植入物上进行实验。打印了一个定义明确的、直角多孔的PLA螺旋形支架,随后涂抹羟基磷灰石(HA)来提高其骨传导性。作为内固定和理想的细胞传送系统,通过体内外试验对装满间充质干细胞(MSCs)的成骨支架进行评估,来观察经细胞疗法后骨韧带愈合情况。悬浮在PLA/HA 螺钉状的支架上的Pluronic F-127水凝胶里的MSCs显示最高的细胞增殖和体外成骨。体内在4周和12周时对兔模型的前交叉韧带进行评估,结果显示装满悬浮在Pluronic F-127水凝胶里的MSCs的PLA/HA 螺钉状支架表现出显著的骨长入和在骨隧道里骨移植界面形成。总之,该研究结果表明,在诊所(fab@clinic)使用D3DPs制作外科植入物是可行、有效和经济的。

 

 

由MediCool医库软件 沈磊 编译

原文来自:Sci Rep